不同毒力结核分枝杆菌对THP-1细胞凋亡及细胞因子表达的影响

doi:10.11843/j.issn.0366-6964.2015.09.015

Abstract

Abstract:

In order to investigate the relationship between apoptosis and transcription and expression of several major cytokines during Mycobacterium tuberculosis (MTB)infection，THP-1 derived macrophage was selected to establish in vitro model.Infected cells with different virulence MTB (H37Rv，BCG)，flow cytometry was used to detect macrophage apoptosis at six time points，quantitative PCR was used to detect transcription level of cytokines TNF-α，IL-10，IL-6，ELISA was used to detect the secretion of cell supernatants of three cytokines.The results showed：the early apoptotic rate of BCG and H37Rv group were both higher than control group and the difference was statistically significant （P<0.05）.Transcription levels of cytokines，TNF-α，IL-6 were consistent with the level of secretion.There was an increase with time on apoptosis of low virulent strain，and there is a same trend on secretion of TNF-α，an opposite tendency on secretion of IL-6.Apoptosis of virulent strains was less than attenuated vaccine strain.Secretion of virulent strains was complex，like increase first and then suppression.The relationship between cytokines and apoptosis of cells at different time points may provide new ideas for study of macrophage apoptosis mechanisms.

CLC Number:

S852.618

ZHANG Yan，SONG Ji-wei，ZENG Fan-li，SHI Kun，LI Jian-ming，LIU Yang,LIU Fei，SUN Fan-ting，DU Rui. Effect of Different Virulence Mycobacterium tuberculosis on Cytokine Expression and Apoptosis of THP-1 Cells[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(9): 1600-1605.

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References

［1］YOUNG B D，PERKINS M D，DUNCAN K，et al.Confronting the scientific obstacles to global control of tuberculosis［J］.J Clin Invest，2008，118(4)：1255-1265.
［2］ZANELLA G，BAR-HEN A，BOSCHIROLI M L，et al.Modelling transmission of bovine tuberculosis in red deer and wild boar in Normandy，France［J］.Zoonoses Public Health，2012，59(Suppl 2)：170-178.
［3］NIEMANN S，RICHTER E，RÜSCH-GERDES S.Biochemical and genetic evidence for the transfer of Mycobacterium tuberculosis subsp.caprae Aranaz et al.1999 to the species Mycobacterium bovis Karlson and Lessel 1970(approved lists 1980) as Mycobacterium bovis subsp.caprae comb.nov［J］.Int J Syst Evol Microbiol，2002，52(Pt2)：433-436.
［4］DANNENBERG A M Jr.Perspectives on clinical and preclinical testing of new tuberculosis vaccines［J］.Clin Microbiol Rev，2010，23(4)：781-794.
［5］HARRIS J，HOPE J C，KEANE J.Tumor necrosis factor blockers influence macrophage responses to Mycobacterium tuberculosis［J］.J Infect Dis，2008，198(12)：1842-1850.
［6］WEI J，DAHL J L，MOULDER J W，et al.Identification of a Mycobacterium tuberculosis gene that enhances mycobacterial survival in macrophages［J］.J Bacteriol，2000，182(2)：377-384.
［7］SHIN D M，JEON B Y，LEE H M，et al.Mycobacterium tuberculosis eis regulates autophagy，inflammation，and cell death through redox-dependent signaling［J］.PLoS Pathog，2010，6(12)：e1001230.
［8］KIM K H，AN D R，SONG J，et al.Mycobacterium tuberculosis Eis protein initiates suppression of host immune responses by acetylation of DUSP16/MKP-7［J］.Proc Natl Acad Sci U S A，2012，109(20)：7729-7734.
［9］ZHU J，GUO L，MIN B，et al.Growth factor independent-1 induced by IL-4 regulates Th2 cell proliferation［J］.Immunity，2002，16(5)：733-744.
［10］COOPER A M.Cell-mediated immune responses in tuberculosis［J］.Annu Rev Immunol，2009，27：393-422.
［11］杨颖乔，彭圣威.肺结核患者细胞因子水平测定的临床研究［J］.咸宁学院学报(医学版)，2009，23（3）：211-212.
YANG Y Q，PENG S W.Clinical research of cytokine levels on tuberculosis patients［J］.Journal of Xianning University (Medical sciences)，2009，23(3)：211-212.(in Chinese)
［12］DUTTA R K，KATHANIA M，RAJE M，et al.IL-6 inhibits IFN-γ induced autophagy in Mycobacterium tuberculosis H37Rv infected macrophages［J］.Int J Biochem Cell Biol，2012，44(6)：942-954.
［13］卜文甲．家畜结核分枝杆菌致病性的分析［J］.养殖技术顾问，2012(11)：148.
BU W J.Analysis of the pathogenic on livestock Mycobacterium tuberculosis［J］.Technical Advisor for Animal Husbandry，2012(11)：148.(in Chinese)
［14］GRIFFIN J F，RODGERS C R，LIGGETT S，et al．Tuberculosis in ruminants：characteristics of intra-tonsilar Mycobacterium bovis infection models in cattle and deer［J］．Tuberculosis(Edinb)，2006，86(6)：404-418.
［15］JANSEN F H，ROOBOL M，JENSTER G，et al．Screening for prostate cancer in 2008 II：the importance of molecular subforms of prostate-specific antigen and tissue kallikreins［J］.Eur Urol，2009，55(3)：563-574．
［16］BALCEWICZ-SABLINSKA M K，KEANE J，KORNFELD H，et al.Pathogenic Mycobacterium tuberculosis evades apoptosis of host macrophages by release of TNF-R2，resulting in inactivation of TNF-α［J］. J Immunol，1998，161(5)：2636-2641.

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Effect of Different Virulence Mycobacterium tuberculosis on Cytokine Expression and Apoptosis of THP-1 Cells

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